At the moment, it uses an internal database. A scan function (available both in the Sequence Window and in the Toolbar) to automatically detect Features.A new panel in the Sequence Window to manage Features/Annotations.Features can be added manually or by scanning (see bellow). Serial Cloner now handles Features/Annotations.Finally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server and extract a consensus a virtual cutter, a web browser with instant import of NCBI/EMBL entries and a silent restriction map generator.Serial Cloner also allows to quickly prepare Fasta-formatted text or files to submit a multi-alignement request to Clustal. Just select, blunt if you need, and click the Ligate button.An additional interface allows easy Gateway cloning for both BP and LR reactions. shRNA constructions based on pre-defined scaffolds are also automated.You can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. In addition to the classical restriction maps - both graphic and text-based - or site usage windows, you will be able to quickly set-up a PCR-based amplification or prepare synthetic adaptors. The graphic map of Serial Cloner is really Graphic as you can easily select and extract a feature, a fragment or show single, double or multiple cutter all in the same window.Serial Cloner will assist you in setting-up new sub-cloning projects and in preparing the electronic versions of your constructs. All key functions are accessible through a compact interface.Serial Cloner also lets you build text restriction map and quickly format it to add multi-frame translation or only show single cutters for example. All the tools you need to analyze and manipulate your sequences are available in an all-in-one-window concept. Genbank/EMBL files can also be imported or read from internet using the included web interface.Powerful graphical display tools and simple interfaces help the analysis and construction steps in a very intuitive way. Also new in this version is the possibility to read ApE and VectorNTI files with their features as well as pDRAW32 sequences. User can annotate any sequence portion or use the automatic scan to detect common features.Serial Cloner can read and write DNA Strider-compatible files and also import or export files in FASTA format.
ALTERNATIVE TO SERIAL CLONER SOFTWARE
Provides tools with an intuitive interface that assists you in DNA cloning, sequence analysis and visualization Serial Cloner is a Molecular Biology software that provides tools with an intuitive interface to assists you in DNA cloning, sequence analysis and visualization.Serial Cloner now handles Features/Annotations, which has been the most requested enhancement.
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